Cloning of designed transgenic vector into E.coli
Vector design and E. coli colony screening
This protocol was created by the Martindale lab.
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Digestion of plasmid and ligation with inserts
Digesting plasmid with restriction enzymes for vector + insert ligation
This protocol was created by the Martindale lab. [Read More] -
Generation of mRNA for injections
Making mRNA from engineered plasmids for injecting N. vectensis embryos
This protocol was created by the Martindale lab. [Read More]