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My lab notebook

Details and protocols related to my research as a Postdoctoral Associate

Bacterial Recombinant Protein Expression

Inducing IDPs expression in E. coli

Posted on July 15, 2024

The pBAD plasmids are pBR322-derived expression vectors designed for regulated, secreted recombinant protein expression and purification in E. coli. [Read More]
Tags:
- protein
- Ecoli
N. vectensis in situ of biomineralization coral genes homologs

In situ hybridization of N. vectensis genes homologous to coral biomineralization-related genes

Posted on May 22, 2024

Available soon
Tags:
- in situ
Purification of coral, sea urchin and mouse acidic proteins from transgenic Nematostella embryos

Using the ÄKTA go - FPLC

Posted on November 17, 2023

This protocol was designed to isolate coral CARP4, sea urchin SM30 and mouse ameloblastin proteins from transgenic Nematostella vectensis embryos. [Read More]
Tags:
- FPLC
- HisTag
Isolation of mCherryCARP1 protein from Nemtostella vectensis injected embryos

Using the RFP-Trap Agarose Kit

Posted on June 4, 2023

Using the RFP-Trap Agarose Kit on Nemtostella vectensis embryos injected with a plasmid containing the fluorescent protein mCherry bound to the acidic coral protein CARP1. [Read More]
Tags:
- protein isolation
Protein gel electrophoresis

Using the NuPAGE Bis-Tris Mini Gel

Posted on June 3, 2023

Using the NuPAGE Bis-Tris Mini Gel for protein purification experiments on transgenic Nemtostella vectensis embryos. [Read More]
Tags:
- protein
- gel

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